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ion exchange chromatography membranes  (Vivascience Inc)

 
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    Vivascience Inc ion exchange chromatography membranes
    Ion Exchange Chromatography Membranes, supplied by Vivascience Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ion exchange chromatography membranes/product/Vivascience Inc
    Average 90 stars, based on 1 article reviews
    ion exchange chromatography membranes - by Bioz Stars, 2026-06
    90/100 stars

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    FIG. 4. Dephosphorylation of the insulin receptor by trans- membrane PTPases PTP-a and LAR isolated on a Mono Q col- umn. Adipocyte plasma membrane (2–3 mg) was solubilized in 1% Triton X-100 and then loaded onto a 5-cm Mono Q column. Fractions were eluted with a 0.05–1.0 M NaCl gradient using an Amersham Pharmacia Biotech FPLC apparatus. LAR and PTP-a were detected by Western blot with specific antibodies (A), and the arrow indicates the position of LAR (the rightmost lane is LAR from cells transfected as in Ref. 21). Column fractions were pooled, concentrated, and assayed for a PTPase activity with autophosphorylated, 32P-labeled insulin receptor (10 mg of total protein, ;0.1–0.5 mg of receptor) as a substrate (B). The results are representative of two separate experiments.

    Journal: The Journal of biological chemistry

    Article Title: Dynamics of protein-tyrosine phosphatases in rat adipocytes.

    doi: 10.1074/jbc.275.9.6308

    Figure Lengend Snippet: FIG. 4. Dephosphorylation of the insulin receptor by trans- membrane PTPases PTP-a and LAR isolated on a Mono Q col- umn. Adipocyte plasma membrane (2–3 mg) was solubilized in 1% Triton X-100 and then loaded onto a 5-cm Mono Q column. Fractions were eluted with a 0.05–1.0 M NaCl gradient using an Amersham Pharmacia Biotech FPLC apparatus. LAR and PTP-a were detected by Western blot with specific antibodies (A), and the arrow indicates the position of LAR (the rightmost lane is LAR from cells transfected as in Ref. 21). Column fractions were pooled, concentrated, and assayed for a PTPase activity with autophosphorylated, 32P-labeled insulin receptor (10 mg of total protein, ;0.1–0.5 mg of receptor) as a substrate (B). The results are representative of two separate experiments.

    Article Snippet: Purification of PTPases by a Mono-Q Fast Performance Liquid Chromatography (FPLC) Ion Exchange Chromatography—Solubilized plasma membrane (2–3 mg of protein) was subjected to FPLC with a Mono Q column (HR5/5; Amersham Pharmacia Biotech).

    Techniques: De-Phosphorylation Assay, Membrane, Isolation, Clinical Proteomics, Western Blot, Transfection, Activity Assay, Labeling